human peripheral blood lymphocyte separation medium (Beijing Solarbio Science)

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Beijing Solarbio Science human peripheral blood lymphocyte separation medium
Human Peripheral Blood Lymphocyte Separation Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+peripheral+blood+lymphocyte+separation+medium+lymphocyte+separation+medium+(human)/Lymphocyte+Separation+Medium/pm41876831-352-13-19
Average 96 stars, based on 631 article reviews

human peripheral blood lymphocyte separation medium - by Bioz Stars, 2026-09

96/100 stars

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Isolation:

Article Title: TRIM21-mediated degradation of HILPDA overcomes anti-PD-1 immunotherapy resistance in breast cancer by limiting PD-L1 palmitoylation
Article Snippet: .. Peripheral blood samples were collected from healthy donors, and PBMCs were isolated using human peripheral blood lymphocyte separation medium (Solarbio, China). .. The isolated PBMCs were cultured in complete RPMI-1640 medium (GIBCO, USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin–streptomycin (GIBCO, USA).

Article Title: Engineered RGD‐Treg‐Exos Targeted Delivery of miR‐218‐5p to Activate Mitophagy and Attenuate Podocyte Injury in Diabetic Kidney Disease
Article Snippet: .. Peripheral blood from healthy controls was isolated using a lymphocyte separation medium (Solarbio, Beijing, China) to generate single‐cell suspensions. .. A human CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) was used to isolate CD4+CD25+Treg cells, according to the instruction manual.

Article Title: Multi-omics analysis reveals the association of cesarean delivery with altered gut microbial profiles and a Th2-biased immune response in neonates
Article Snippet: .. The remaining blood was used for PBMC isolation via human peripheral blood lymphocyte separation medium (P8610, Solarbio, China). ..

Article Title: High-dose vitamin C potently induces apoptosis in acute lymphoblastic leukemia by activating ER stress response.
Article Snippet: .. Mononuclear cells were isolated using human peripheral blood lymphocyte separation medium (Lymphocyte Separation Medium (Human) from Solarbio). ..

Article Title: TRIM21-mediated degradation of HILPDA overcomes anti-PD-1 immunotherapy resistance in breast cancer by limiting PD-L1 palmitoylation.
Article Snippet: .. Peripheral blood samples were collected from healthy donors, and PBMCs were isolated using human peripheral blood lymphocyte separation medium (Solarbio, China). .. The isolated PBMCs were cultured in complete RPMI-1640 medium (GIBCO, USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin–streptomycin (GIBCO, USA).

Adjuvant:

Article Title: Isolation of an Anti-hG-CSF Nanobody and Its Application in Quantitation and Rapid Detection of hG-CSF in Pharmaceutical Testing
Article Snippet: .. Freund’s complete adjuvant (FCA) and Freund’s incomplete adjuvant (FIA) (F5881 and F5506, Merck, Billerica, MA, USA), Goat anti-Alpaca IgG(H+L)-HRP (053-404-005, AlpVHHs, Chengdu, China), Human Peripheral Blood Lymphocyte Separation Medium (P8610, Solarbio, Beijing, China), TRIzol Plus RNA Purification Kit (12183555, Invitrogen, Carlsbad, CA, USA), SuperScript III Reverse Transcriptase (18080093, Invitrogen, Carlsbad, CA, USA), DNA Gel Extraction Kit (NA1111, Merck, Billerica, MA, USA), Hot Start Taq DNA Polymerase (M0490S, NEB, Beverly, MA, USA), DNA Ligase (M2622S, NEB, Beverly, MA, USA), restriction enzymes NotI, SfiI, MscI, and XhoI (R0189S, R0123S, R0534S, and R0146S, NEB, Beverly, MA, USA), M13KO7 helper phage (N0315S, NEB, Beverly, MA, USA), PEG-NaCl solution (C7052, Bioss, Beijing, China), carbonate–bicarbonate buffer (C3041-50CAP, Merck, Billerica, MA, USA), ELISA microplate (154041, Thermo Scientific, Waltham, MA, USA), TMB Liquid-1 component (J644, Amresco, Solon, OH, USA), BL21 (DE3) competent cells (EC0114, Thermo Scientific, Waltham, MA, USA), isopropyl β-D-thiogalactopyranoside (IPTG, I5502, Merck, Billerica, MA, USA), High-Performance HisTrap column (GE17-5248-01, Cytiva, Marlborough, MA, USA), imidazole (I5513, Merck, Billerica, MA, USA), NuPAGE precast polyacrylamide gel (NP0321BOX, Invitrogen, Carlsbad, CA, USA), DAB Substrate Kit (SK-4105, Vector Laboratories, Burlingame, CA, USA), Coomassie Brilliant Blue (P0017B, Beyotime, Shanghai, China), G-CSF monoclonal antibody (sc-53292, SANTA CRUZ Biotechnology, Santa Cruz, CA, USA). ..

Purification:

Reverse Transcription:

Gel Extraction:

Enzyme-linked Immunosorbent Assay:

Cell Culture:

Article Title: A small molecule directly targets NLRP3 to promote inflammasome activation and antitumor immunity
Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) from the peripheral blood of healthy people were obtained using the Human Lymphocyte Separation Medium kit (Solarbio), and then cultured in Roswell Park Memorial Institute (RPIM)-1640 containing 10% FBS and 1% antibiotics. ..

Review